Fredericamycin derivatives

ABSTRACT

The invention relates to novel fredericamycin derivatives, to medicaments containing these derivatives or salts thereof, and to the use of fredericamycin derivatives for treating diseases, particularly tumor diseases.

The invention relates to novel fredericamycin derivatives, to drugs containing said derivatives or the salts thereof, and to the use of the fredericamycin derivatives for treating diseases, particularly tumor diseases.

Fredericamycin has been isolated 1981 from Streptomyces griseus, and demonstrates anti-tumor activity.

Fredericamycin and several fredericamycin derivatives are known.

In Heterocycles 37 (1994) 1893-1912, J. Am. Chem. Soc. 116 (1994) 9921-9926, J. Am. Chem. Soc. 116 (1994) 11275-11286, J. Am. Chem. Soc. 117 (1995) 11839-11849, JP 2000-072752, and in J. Am. Chem. Soc. 123 (2001), various total syntheses of fredericamycin A have been described, some being enantio-selective.

In U.S. Pat. No. 4,673,768, alkali salts of the fredericamycin A are described. In U.S. Pat. No. 4,584,377, fredericamycin derivatives are described, particularly derivatives acylated in ring E and F. In U.S. Pat. No. 5,166,208, fredericamycin derivatives are described as well, particularly derivatives carrying thio and amino substituents in ring F. The derivatives are generated semi-synthetically or fully synthetically.

Surprisingly it was found that fredericamycin derivatives, especially those derivatized in ring A, represent potent drugs. Also, a possibility was found to introduce such residues in ring A semi-synthetically, with which the water solubility, among others, can be significantly increased. Other derivatisation methods known from the art can also be performed with the derivatives according to the invention. Furthermore, an alternative method was found to make fredericamycin derivatives water-soluble by generating cyclodextrin inclusion compounds.

The invention relates to novel fredericamycin derivatives with the general Formula Ia or Ib:

wherein in each,

-   R1 means H, C₁-C₆ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl, -   R2 means C₁-C₁₄ alkyl, C₂-C₁₄ alkenyl, 1,3-butadienyl, 1-butane,     C₁-C₄ alkylaryl, heteroaryl, C₁-C₄ alkyl heteroaryl, cycloalkyl,     C₁-C₄ alkyl-cycloalkyl, heterocycloalkyl, C₁-C₄     alkylheterocycloalkyl, C_(m)H_(2m+o−p)Y″_(p) (with m=1 to 6, for     o=1, p=1 to 2m+o; for m=2 to 6, o=−1, p=1 to 2m+o; for m=4 to 6,     o=−2, p=1 to 2m+o; Y″=independently from each other selected from     the group consisting of halogen, OH, OR21, NH₂, NHR21, NR21R22, SH,     SR21), CH₂NHCOR21, CH₂NHCSR21, CH₂S(O)nR21, with n=0, 1, 2,     CH₂SCOR21, CH₂OSO₂—R21, CHO, CH═NOH, CH(OH)R21, —CH═NOR21,     —CH═NOCOR21, —CH═NOCH₂CONR21R22, —CH═NOCH(CH₃)CONR21R22,     —CH═NOC(CH₃)₂CONR21R22, —CH═N—NHCO—R23, —CH═N—NHCO—CH₂NHCOR21,     —CH═N—O—CH₂NHCOR21, —CH═N—NHCS—R23, —CH═CR24R25 (trans or cis),     COOH, COOR21, CONR21R22, —CH═NR21, —CH═N—NR21R22,

with X′=NR215, O, S, and R211, R212, R213, R214, R215 being independently from each other H or C₁-C₆ alkyl), —CH═N—NHSO₂ aryl, —CH═N—NHSO₂-heteroaryl,

-   R21, R22 are independently from each other C₁-C₁₄ alkyl, C₁-C₁₄     alkanoyl, C₁-C₆ alkylhydroxy, C₁-C₆ alkylamino, C₁-C₆     alkylamino-C₁-C₆ alkyl, C₁-C₆ alkylamino-di-C₁-C₆ alkyl, cycloalkyl,     C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄     alkylheterocycloalkyl, aryl, aryloyl, C₁-C₄ alkylaryl, heteroaryl,     heteroaryloyl, C₁-C₄ alkylheteroaryl, cycloalkanoyl, C₁-C₄     alkanoylcycloalkyl, heterocycloalkanoyl, C₁-C₄     alkanoylheterocycloalkyl, C₁-C₄ alkanoylaryl, C₁-C₄     alkanoylheteroaryl, mono- and di-sugar residues linked through a C     atom which would carry an OH residue in the sugar, wherein the     sugars are independently from each other selected from the group     consisting of glucuronic acid and its stereo isomers at all optical     atoms, aldopentoses, aldohexoses, including their desoxy compounds     (such as e.g. glucose, desoxyglucose, ribose, desoxyribose), -   R23 independently of R21, has the same meanings as R21, or     CH₂-pyridinium salts, CH₂-tri-C₁-C₆ alkylammonium salts, -   R24 independently of R21, has the same meanings as R21, or H, CN,     COCH₃, COOH, COOR21, CONR21R22, NH₂, NHCOR21, -   R25 independently of R21, has the same meanings as R21, or H, CN,     COCH₃, COOH, COOR21, CONR21 R22, NH₂, NHCOR21, -   R24, R25 together mean C₄-C₈ cycloalkyl, -   R3 means C₂-C₁₄ alkyl, C₂-C₁₄ alkenyl, C₂-C₁₄ alkinyl, aryl, C₁-C₄     alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, wherein the aryls or     heteroaryls may be substituted with another aryl, C₁-C₄ alkylaryl,     O-aryl, C₁-C₄ alkyl-O-aryl, heteroaryl, C₁-C₄ alkylheteroaryl,     O-heteroaryl or C₁-C₄ alkyl-O-heteroaryl, cycloalkyl, C₁-C₄     alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl,     C_(m)H_(2m+o−p)Y′_(p) (with m=2 to 6, for o=1, −1, p=1 to 2m+o; for     m=4 to 6, o=−3, p=1 to 2m+o; Y′=independently from each other     selected from the group consisting of halogen, OH, OR31, NH₂, NHR31,     NR31R32, SH, SR31), CH₂NHCOR31, CH₂NHCSR31, CH₂S(O)nR31, with n=0,     1, 2, CH₂SCOR31, CH₂OSO₂—R31, CHO, CH═NOH, CH(OH)R31, —CH═NOR31,     —CH═NOCOR31, —CH═NOCH₂CONR31R32, —CH═NOCH(CH₃)CONR31R32,     —CH═NOC(CH₃)₂CONR31R32, —CH═N—NHCO—R33, —CH═N—NHCO—CH₂NHCOR31,     —CH═N—O—CH₂NHCOR31, —CH═N—NHCS—R33, —CH═CR34R35 (trans or cis),     COOH, COOR31, CONR31R32, —CH═NR31, —CH═N—NR31R32,

(with X′=NR315, O, S, and R311, R312, R313, R314, R315 being independently from each other H or C₁-C₆ alkyl), —CH═N—NHSO₂-aryl, —CH═N—NHSO₂-heteroaryl,

-   R31, R32 mean independently from each other C₁-C₁₄ alkyl, C₁-C₁₄     alkanoyl, C₁-C₆ alkylhydroxy, C₁-C₆ alkylamino,     C₁-C₆alkylamino-C₁-C₆ alkyl, C₁-C₆ alkylamino-di-C₁-C₆ alkyl,     cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄     alkylheterocycloalkyl, aryl, aryloyl, C₁-C₄ alkylaryl, heteroaryl,     heteroaryloyl, C₁-C₄ alkylheteroaryl, cycloalkanoyl, C₁-C₄     alkanoylcycloalkyl, heterocycloalkanoyl, C₁-C₄     alkanoylheterocycloalkyl, C₁-C₄ alkanoylaryl, C₁-C₄     alkanoylheteroaryl, mono- and di-sugar residues linked through a C     atom which would carry an OH residue in the sugar, wherein the     sugars are independently from each other selected from the group     consisting of glucuronic acid and its stereo isomers at all optical     atoms, aldopentoses, aldohexoses, including their desoxy compounds     (such as e.g. glucose, desoxyglucose, ribose, desoxyribose), -   R33 independently of R31, has the same meanings as R31, or     CH₂-pyridinium salts, CH₂-tri-C₁-C₆ alkylammonium salts, -   R34 independently of R21, has the same meanings as R31, or H, CN,     COCH₃, COOH, COOR21, CONR31R32, NH₂, NHCOR31, -   R35 independently of R31, has the same meanings as R31, or H, CN,     COCH₃, COOH, COOR31, CONR31R32, NH₂, NHCOR31, -   R34, R35 together mean C₄-C₈ cycloalkyl, -   R5 means H, C₁-C₆ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl,     heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, aryl, C₁-C₄     alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, -   R4, R6, R7 independently from each other mean H, C₁-C₆ alkyl,     CO—R41, -   R41 independently of R21, has the same meanings as R21, -   X means O, S, NH, N—R8, wherein R8 independently from R5 may adopt     the same meaning as R5, or R5 and R8, together with the N, form a     ring with 4, 5, 6, 7, or 8 members, which may optionally contain     still another heteroatom selected from the group N, O, S,     or X-R5 may together be H, -   Y means O, S, NR9, wherein R9 may be H or C₁-C₆ alkyl,     as well their stereoisomers, tautomers, and their physiologically     tolerable salts or inclusion compounds.

Preferred are compounds of Formula IIa or IIb

wherein the meaning of the residues R1-R41, X is as described above, their tautomers and their physiologically tolerable salts or inclusion compounds.

The invention further relates to compounds of Formulas Ia, Ib, IIa or IIb, in which the residues R, except for R3, have the above described meanings, and the water solubility of R3 is at least two times higher, preferably at least five timer higher, more preferred at least ten times higher, especially preferred at least fifty time higher, particularly one hundred times higher, or even five hundred times higher than of R3 being H, when all other residues are maintained. The increase in the water solubility is achieved e.g. by introduction of groups which can increasingly form hydrogen bonds, and/or are polar, and/or are ionic. Residues R3 with increased water solubility und with the meaning indicated in the formulas are preferred.

The invention also relates to compounds of the Formula Ia, Ib, IIa or IIb, in which the residues R, except R2, have the above described meanings, and, additionally, the water solubility of R2 is at least two times higher, preferably at least five timer higher, more preferred at least ten times higher, especially preferred at least fifty time higher, particularly one hundred times higher, or even five hundred times higher than of R2 being CH═CH—CH═CH—CH₃, when all other residues are maintained. The increase in water solubility is mediated e.g. by introduction of groups which can increasingly form hydrogen bonds and/or are polar and/or ionic. A key intermediate are compounds with an aldehyde function in R2. Residues R2 with increased water solubility and with the meaning indicated in the Formulas are preferred. Especially preferred are derivatives with increased water solubility in R2 and R3.

Preferred R2 residues are heteroaryl, cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, C_(m)H_(2m+o−p)Y″_(p) (with m=1 to 6, for o=1, p=1 to 2m+o; for m=2 to 6, o=−1, p=1 to 2m+o; for m=4 to 6, o=−2, p=1 to 2m+o; Y″=independently selected from each other from the group of halogen, OH, OR21, NH₂, NHR21, NR21R22, SH, SR21), CH₂NHCOR21, CH₂NHCSR21, CH₂S(O)nR21, with n=0, 1, 2, CH₂SCOR21, CH₂OSO₂—R21, CH(OH)R21, —CH═NOCOR21, —CH═NOCH₂CONR21R22, —CH═NOCH(CH₃)CONR21R22, —CH═NOC(CH₃)₂CONR21R22, —CH═N—NHCO—R23, —CH═N—NHCO—CH₂NHCOR21, —CH═N—O—CH₂NHCOR21, —CH═N—NHCS—R23, —CH═CR24R25 (trans or cis), CONR21R22, —CH═NR21, —CH═N—NR21R22,

(with X′=NR215, O, S, and R211, R212, R213, R214, R215 being independently from each other H or C₁-C₆ alkyl), —CH═N—NHSO₂ aryl, —CH═N—NHSO₂ heteroaryl.

Furthermore preferred are still compounds as described above, wherein the residues R preferably independently from each other adopt one or more of the following meanings:

-   R1 means H, C₁-C₅ alkyl, cycloalkyl, especially H, -   R2 means C₁-C₅ alkyl, C₁-C₄ alkylaryl, C₂-C₅alkenyl, heteroaryl,     C₁-C₄ alkylheteroaryl, CHF₂, CF₃, polyol side chain, particularly     CHOH—CHOH—CHOH—CHOH—CH₃, CHOH—CHOH—CH═CH—CH₃, CH═CH—CHOH—CHOH—CH₃,     CH₂Y′″(Y′″=F, Cl, Br, I), CH₂NH₂, CH₂NR21R22, CH₂NHCOR23,     CH₂NHCSR23, CH₂SH, CH₂S(O)nR21, with n=0, 1, 2, CH₂SCOR21,     particularly CH₂OH, CH₂OR21, CH₂OSO₂—R21, particularly CHO,     CH(OR21)₂, CH(SR21)₂, CN, CH═NOH, CH═NOR21, CH═NOCOR21,     CH═N—NHCO—R23, CH═CR24, R25 (trans or cis), particularly COOH     (particularly their physiologically tolerable salts), COOR21,     CONR21R22, —CH═NR21, —CH═N—NR21R22,

(with X′=NR215, O, S, and R211, R212, R213, R214, R215 being independently from each other H or C₁-C₆ alkyl), —CH═N—NHSO₂ aryl, —CH═N—NHSO₂ heteroaryl, CH═N—NHCO—R23,

-   R21, R22 independently from each other mean C₁-C₆ alkyl, cycloalkyl,     aryl, C₁-C₄ alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, -   R23 independently of R21, has the same meanings as R21, or     CH₂-pyridinium salts, CH₂-tri-C₁-C₆ alkylammonium salts, -   R24 independently of R21, has the same meanings as R21, or H, CN,     COCH₃, COOH, COOR21, CONR21R22, NH₂, NHCOR21, -   R25 independently of R21, has the same meanings as R21, or H, CN,     COCH₃, COOH, COOR21, CONR21R22, NH₂, NHCOR21, -   R24, R25 together mean C₄-C₈ cycloalkyl, -   R3 means C₂-C₁₄ alkyl, C₂-C₁₄ alkenyl, C₂-C₁₄ alkinyl, aryl, C₁-C₄     alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, wherein the aryls or     heteroaryls may be substituted with another aryl, C₁-C₄ alkylaryl,     O-aryl, C₁-C₄ alkyl-O-aryl, heteroaryl, C₁-C₄ alkylheteroaryl,     O-heteroaryl or C₁-C₄ alkyl-O-heteroaryl, -   R5 means H, C₁-C₃ alkyl, cycloalkyl, -   R4, R6, R7 independently from each other mean H, C₁-C₅ alkyl,     CO—R41, -   R41 independently of R21, has the same meanings as R21, -   X means O, S, NH, N—R8, particularly O, -   Y means O, S, NH, particularly O,     their stereoisomers, tautomers, and their physiologically tolerable     salts or inclusion compounds.

Most preferred are the compounds, the stereo isomers, tautomers, and physiologically tolerable salts or inclusion compounds of which, selected from the group consisting of the compounds of the examples 7-10, and the compounds demonstrating combinations of the various substituents of the compounds of these examples.

Also preferred are drugs which contain the above compounds of Formula I or II in addition to the usual carriers and adjuvants.

Also preferred are the above mentioned drugs in combination with other agents for tumor treatment.

These compounds according to the invention are used for preparation of drugs for treatment of tumors, particularly such that may be treated by inhibition of the topoisomerases I and/or II. Tumors that can be treated with the substances according to the invention are e.g. leukemia, lung cancer, melanomas, prostate tumors and colon tumors.

Furthermore, the compounds according to the invention can be used for preparation of drugs for treatment of neurodermitis, parasites and for immunosuppression.

In the description and the claims the substituents are described by the following definitions:

The term “alkyl” by itself or as part of another substituent means a linear or branched alkyl chain radical of the respectively indicated length, in which optionally a CH₂ group may be substituted by a carbonyl function. Thus, C₁₋₄ alkyl may be methyl, ethyl, 1-propyl, 2-propyl, 2-methyl-2-propyl, 2-methyl-1-propyl, 1-butyl, 2-butyl, C₁₋₆ alkyl, e.g. C₁₋₄ alkyl, pentyl, 1-pentyl, 2-pentyl, 3-pentyl, 1-hexyl, 2-hexyl, 3-hexyl, 4-methyl-1-pentyl, or 3,3-dimethylbutyl.

The term “C₁-C₆ alkylhydroxy” by itself or as part of another substituent means a linear or branched alkyl chain radical of the respectively indicated length, which may be saturated or unsaturated, and which carries an OH group, e.g. hydroxymethyl, hydroxymethyl, 1-hydroxypropyl, 2-hydroxypropyl.

The term “alkenyl” by itself or as part of another substituent means a linear or branched alkyl chain radical with one or more C═C double bonds of the respectively indicated length, several double bonds being preferably conjugated. Thus, C₂₋₆ alkenyl may for example be ethenyl, 1-propenyl, 2-propenyl, 2-methyl-2-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2-butenyl, 1,3-butdienyl, 2,4-butdienyl, 1-pentenyl, 2-pentenyl, 3-pentenyl, 1,3-pentdienyl, 2,4-pentdienyl, 1,4-pentdienyl, 1-hexenyl, 2-hexenyl, 1,3-hediexyl, 4-methyl-1-pentenyl, or 3,3-dimethylbutenyl.

The term “alkinyl” by itself or as part of another substituent means a linear or branched alkyl chain radical with one or more C—C triple bonds of the respectively indicated length, wherein additional double bonds may be present as well. Thus, C₂₋₆ alkinyl may for example be ethinyl, 1-propinyl, 2-propinyl, 2-methyl-2-propinyl, 2-methyl-1-propinyl, 1-butinyl, 2-butinyl, 1-pentinyl, 2-pentinyl, 3-pentinyl, 1,4-pentdiinyl, 1-pentine-4-enyl, 1-hexinyl, 2-hexinyl, 1,3-hexdiinyl, 4-methyl-1-pentinyl, or 3,3-dimethylbutinyl.

The term “halogen” stands for fluorine, chlorine, bromine, iodine, preferably bromine and chlorine.

The term “NR21R22”, or analogous NR×1R×2, preferably stand for a dialkylamino group, wherein the two alkyl groups together with the N can form a ring with 5 or 6 members with optionally one more heteroatom N or O.

The term “cycloalkyl” by itself or as part of another Substituent comprises saturated, cyclic carbohydrate groups with 3 to 8 C atoms, such as e.g. cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, 4-methylcyclohexyl, cyclohexylmethylene, cycloheptyl or cyclooctyl.

The term “heterocycloalkyl” by itself or as part of another substituent includes cycloalkyl groups, wherein up to two CH₂ groups may be substituted by oxygen, sulfur or nitrogen atoms, and another CH₂ group may be substituted by a carbonyl function, for example pyrrolidine, piperidine, morpholine or

The term “aryl” by itself or as part of another substituent includes aromatic ring systems with up to 3 rings, in which at least 1 ring system is aromatic, and those with up to 3 substituents, preferably up to 1 substituent, wherein the substituents independently from each other can have the meaning C₁-C₆ alkyl, OH, NO₂, CN, CF₃, OR11, SH, SR11, C₁-C₆ alkylhydroxy, C₁-C₆ alkyl-OR11, COOH, COOR11, CONH2, CONR11R12, CHO, CH═NO—C₁-C₁₀ alkyl, C₁l-C₁₀ alk-1-enyl, NH₂, NHR11, NR11R12, halogen, wherein the residues R11 und R12 independently from each other can mean C₁-C₁₀ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl.

Apart from phenyl and 1-naphthyl and 2-naphthyl, preferred aryls are:

The term “heteroaryl” by itself or as part of another substituent includes aromatic ring systems with up to 3 rings and with up to 3 identical or different heteroatoms N, S, O, in which at least 1 ring system is aromatic, and those with up to 3 substituents, preferably up to 1 substituent, wherein the substituents independently from each other can have the meaning C₁-C₆ alkyl, OH, NO₂, CN, CF₃, OR11, SH, SR11, C₁-C₆ alkylhydroxy, C₁-C₆ alkyl-OR11, COOH, COOR11, CONH₂, CONR11R12, CHO, CH═NO—C₁-C₁₀ alkyl, C₁-C₁₀ alk-1-enyl, NH₂, NHR11, NR11R12, halogen, wherein the residues R11 und R12 independently from each other can mean C₁-C₁₀ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl.

Preferred heteroaryls are:

Particularly preferred are 2-furyl, 3-furyl, 2-thiophenyl, 3-thiophenyl, 3-pyridinyl, 4-pyridinyl, 4-isoxazolyl, 2-N-methylpyrrolyl, and 2-pyrazinyl. Especially preferred are these as residues R3.

The term “ring system” generally refers to rings with 3, 4, 5, 6, 7, 8, 9, or 10 members. Preferred are rings with 5 and 6 members. Furthermore, ring systems with one or 2 annelated rings are preferred.

The compounds of Formula I may be present as such, or, if they contain acidic or basic groups, in the form of their salts with physiologically tolerable bases or acids. Examples for such acids are: hydrochloric acid, citric acid, trifluoracetic acid, tartaric acid, lactic acid, phosphoric acid, methane sulfonic acid, acetic acid, formic acid, maleic acid, fumaric acid, succinic acid, hydroxysuccinic acid, sulfuric acid, glutaric acid, aspartic acid, pyruvic acid, benzoic acid, glucuronic acid, oxalic acid, ascorbic acid, and acetylglycine. Examples for bases are alkali ions, preferably Na, K, alkaline earth ions, preferably Ca, Mg, ammonium ions.

The compounds according to the invention may be administered orally in the usual way. The application may also be i.v., i.m., with vapors, or sprays through the nasopharynx.

The dosage depends on age, condition and weight of the patient as well as on the type of application. Usually, the daily dose of the active ingredient per person is between 0.1 μg/kg and 1 g/kg orally. This dosage may be given as 2 to 4 split dosages, or once per day as a slow release form.

The novel compounds may be used in the usual solid or liquid pharmaceutical application forms, e.g. as tablets, film tablets, capsules, powder, granules, coated tablets, solutions, or sprays. These are prepared in the usual way. The agents can be processed with the usual pharmaceutical adjuvants such as tablet binders, fillers, preservatives, disintegrants, flow regulators, plasticizers, wetting agents, dispersants, emulsifiers, solvents, retardation agents, antioxidants, and/or propellants (see H. Sucker et al.: Pharmazeutische Technologie, Thieme-Verlag, Stuttgart, 1978). Usually, the so obtained application forms contain the active ingredient in amounts of 0.1 to 99 percent per weight.

Experimental Part

Fredericamycin A can be prepared by fermentation or fully synthetically according to the known methods. The reduced forms of the Formulas Ib and IIb can be obtained from the appropriate compounds of Formulas Ia and IIa using mild reducing agents.

PREPARATION OF THE SUBSTANCES Substitution at the B ring Palladium-catalyzed C—C bond

Fredericamycin (1) can be reacted with halogenization agents such as N-bromosuccinimide (NBS) and N-iodosuccinimide (NIS) to the 5-bromo- or 5 iodofredericamyin derivatives (2) and (3) with good yields (diagram 1).

By palladium-catalyzed cross couplings according to Suzuki, Stille or according to Heck, with organoboron compounds or stannous compounds such as, e.g. trans-1-hexene-1yl-boronic acid (4), phenylboronic acid (5), and 4-fluorophenylboronic acid (6), the appropriate C—C-linked fredericamycin derivatives (7), (8) and (9) are accessible (see diagram 2).

Also, derivatives with X equaling an aldehyde function can be prepared according to diagrams 3 and 4. For example for the sequence X equaling 1) Br, 2) pentadienyl, 3) tetrol, 4) aldehyde. The other derivatizations according to the inventions are then possible through the aldehyde function.

For the synthesis of further water soluble fredericamycin derivatives, fredericamycin (1) was first hydroxylated with osmium(IV)oxide at the diene side chain.

The fredericamyin tetrol (10) also serves as an important intermediate for the synthesis of the herein mentioned fredericamyin derivatives with increased solubility and/or efficacy profile. By iodine cleavage with sodium metaperiodate or carrier-bound periodate, the tetrol side chain can be broken down to the fredericamycin aldehyde (11) with very high yields (see diagram 4).

This aldehyde may be reacted by bromating reagents such as N-bromosuccinimide, bromine or other bromine generating reagents to a compound that is bromated in the nucleus (12) (see diagram 5).

Surprisingly it also was shown, that fredericamycin aldehyde iodated in the nucleus (13) is generated in one step of the above described diol cleavage [(10)→(11)]. This surprising reaction is only observed, if dimethylsulfoxide (DMSO) is used as a solvent instead of dimethylformamide (DMF) (see diagram 6).

Both, the iodized fredericamycin aldehyde (13) and the bromated fredericamycin aldehyde (12) are suitable for the generation of substance libraries.

As an example of a substance library, the aldehyde (12) may be reacted to the appropriate R3-substituted oximes, e.g. with hydroxylamines and hydrazines and a subsequent Pd-catalyzed C—C coupling (see diagram 7).

In the following diagrams, fredericamycin and its derivatives are used to show how analogous derivatives according to the invention can be prepared.

The compound (24) is the precursor of an N-methylated fredericamycin derivative (diagram 8).

Fredericamycin may be transformed by palladium/hydrogen almost quantatively to tetrahydro fredericamycin (25), and may be halogenated in the nucleus according to the above described methods, e.g. to the bromine compound (26) (see diagram 9):

Surprisingly it has also been found that the methoxy groups in fredericamycin and the derivatives according to the invention can be exchanged under alkali or earth alkali acetate catalysis by oxygen nucleophiles such as alcohols or polyols. Thereby, the alcohols can carry a multitude of different substituents.

Exchange of the Methoxy Group at the F Ring

The exchange of the methoxy groups at the F ring of the fredericamycin and at the derivatives is possible by primary, secondary or aromatic amines. Thereby, the components are stirred with the appropriate primary or secondary amines at room temperature in DMF or in another inert solvent. With aromatic amines, a catalysis with Lewis acids such as stannous(IV)chloride, etc. is required.

Preparation of Thioanalogoues of Fredericamycin Derivatives

By sulfurization of fredericamycin or its derivatives with Lawesson reagent or P₄S₁₀ in pyridine, the derivatives analogous to thiopyridone are accessible (see diagram 12).

Fredericamycin (1) forms inclusion compounds such as (22) with polysugars such as α-cyclodextrin that have good water solubility compared to the original substance.

The dextrin inclusion compounds form easily if the components are mixed in the appropriate stoichiometric ratio in a suitable solvent such as DMSO.

EXAMPLES Example 1 1-Desoxy-5-C-[(8R)-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-yl]pentitol (10)

Two hundred (200) mg (0.38 mmol) fredericamycin A (1) are dissolved in 30 mL dichloromethane. After addition of 20 mL methanol and 4.4 ml water, 350 mg (2.6 mmol) N-methylmorpholine-N-oxide are added. Under vigorous stirring, 0.2 ml of a 2.5% osinium(IV)oxide solution in t-butanol is added dropwise. The reaction mixture is acidified with 2-3 drops of trifluoracetic acid. After stirring for 48 hours, the reaction is complete according to HPLC control (RP18, acetonitrile water (0.2% acetic acid)). The reaction mixture is added to 400 ml water under vigorous stirring, and the dark red crystalline solid is sucked off through a filter. Drying in HV. Yield: 195 mg (87% of the theoretical value) dark red powder. ES⁻: M/e=606.2 (M+−H), λmax: 504.0.

Example 2 (8S)-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-carbaldehyde (11)

-   1. ) Fifty (50) mg (82.3 μmol) tetrahydroxy fredericamycin (tetrol     (2)) are dissolved in 4 mL DMF. Under vigorous stirring, an aqueous     sodium iodate solution (300 mg NaIO₄ in 1 mL water) is added     dropwise within one hour. After 1 h stirring at room temperature, 2     drops of trifluoracetic acid are added. After stirring for another     30 min, the reaction solution is diluted with 3 ml DMF, and 150 mg     NaIO₄ dissolved in 0.5 ml water are added.

After another hour, 100 mL water are added. The supernatant over the precipitate is sucked off, and dryed in HV. Dark red crystal powder. Yield: 41 mg (100% of the theoretical value). M/e=501.3, UV_(max): 504.0 nm.

-   2. ) One hundred and nine (109) mg (179 μmol) fredericamycin     tetrol (2) are dissolved in 8 mL pyridine. 180 μL water are added.     To the reaction mixture, 450 mg (1.08 mmol, 6 eq.)     (polystryrylmethyl)trimethylammonium periodate resin are added. Then     the mixture is stirred for 12 h at RT. The resin is filtered off;     washing and concentrating until dry. Dark red residue. Yield: 89.9     mg (100% of the theoretical value). M/e=501.3, UV_(max): 504.0 nm.

Example 3 (8S)-5-bromo-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′,3′,5′,8′(2H)-pentone (2)

Twenty (20) mg (37.1 μmol) fredericamycin (1) were dissolved in 250 μl DMF, and then 6.3 mg (35.3 μmol) N-bromosuccinimide in 250 μl DMF were added within one hour at 0° C. The reaction was stirred in a slowly thawing ice bath over night. Then, the DMF is removed in high vacuum, and the residue is purified by preparative HPLC.

Yield: 7 mg (32% of the theoretical value) red crystal mass. M/e=616.1/618.1; λ_(max): 486.0 nm.

Example 4 (8S)-5-iodo-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′,3′,5′,8′(2H)-pentone (3)

Eighty four (84) mg (158 μmol) fredericamycin (1) were dissolved in 1.0 μl DMF, and then 33.0 mg (150.0 μmol) N-iodosuccinimide in 500 μl DMF were added within one hour at 0° C. The reaction was stirred in a slowly thawing ice bath over night. Then, the DMF is removed in high vacuum, and the residue (120 mg (14) with a content of 80%) is purified by preparative HPLC (gradient CH₃CN 50-90% over 16 min.)

Yield: 18 mg (17% of the theoretical value) red crystal mass. M/e=665.0; λ_(max): 484.0 nm.

Example 5 (8S)-4′,9,9′-trihydroxy-5-bromo-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-carbaldehyde (12)

Hundred (100) mg (200 μmol) fredericamycin aldehyde (4) are dissolved under argon in 5 ml DMF. Then, 200 μl of a 1M bromine solution in DMF is added. After stirring for 1.5 h at RT, another 20 μl bromine solution are added. According to HPLC monitoring, the reaction mixture is complete after 3.5 h.

Add to 150 ml water, and shake out with dichloromethane.

Yield: 96 mg (83% of the theoretical value) dark red powder. M/e=579/581; λ_(max): 504.0.

Example 6 (8S)-4′,9,9′-trihydroxy-5-iodo-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-carbaldehyde (13)

Thirty (30) mg (49 μmol) fredericamycin tetrol (10) are dissolved in 1 ml dimethylsulfoxide/water 9/1. TO the reaction mixture 309 mg (2.4 mmol/g, 15 eq.) (polystyrylmethyl)trimethylammoniumperiodate resin are added. Then, the mixture is stirred for 48 h at RT. Then, it is filtered off the resin, diluted with water, and extracted 3× with dichloromethane to which 1% trifluoracetic acid has been added. After drying, it is concentrated until dry. Dark-red residue (HPLC clean). Yield 27.8 mg (90% of the theoretical value), M/e=626.2; UV_(max): 500.0 nm

Example 7 (8S)-5-(trans-1-hexene-1yl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′,3′,5′,8′(2H)-pentone (7)

Ten (10) mg (15 μmol) iodofredericamycin (3) are dissolved in 1 ml DMF under argon, then 4.8 mg (37.5 μmol) trans-1-hexene-1yl-boronic acid (4), 0.9 mg (0.78 μmol) tetrakis(triphenyl)palladium (0) and 75 μl (150 μmol) 2 M Na₂CO₃ solution are added. It is stirred for 1 h at room temperature, and is then heated to 90° C. for 12 h. The reaction mixture is divided between dichloromethane and 1 N hydrochloric acid. The product was purified by preparative HPLC (RP18, CH₃CN—H₂O).

Yield: 4.5 mg (48% of the theoretical value)

Example 8 (8S)-5-phenyl-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′,3′,5′,8′(2H)-pentone (8)

Ten (10) mg (15 μmol) iodofredericamycin (3) are dissolved in 1 ml DMF under argon, then 4.6 mg (37.7 μmol) phenylboronic acid (5), 0.9 mg (0.78 μmol) tetrakis(triphenyl)palladium (0) and 75 μl (150 μmol) 2 M Na₂CO₃ solution are added. It is stirred for 1 h at room temperature, and is then heated to 90° C. for 12 h. The reaction mixture is divided between dichloromethane and 1 N hydrochloric acid. The residue was purified by preparative HPLC (RP18, CH₃CN—H₂O).

Yield: 4.0 mg (43% of the theoretical value), M/e=615.0

Example 9 (8S)-5-(4-fluorophenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′,3′,5′,8′(2H)-pentone (9)

Ten (10) mg (15 μmol) iodofredericamycin (3) are dissolved in 1 ml DMF under argon, then 5.3 mg (37.8 μmol) 4-fluorophenylboronic acid (6), 1.0 mg (0.87 μmol) tetrakis(triphenyl)palladium (0) and 35.2 mg (109 μmol) thallium carbonate are added. It is stirred for 12 h at 90° C. The reaction mixture is divided between dichloromethane and 1 N hydrochloric acid, and its residue was separated by preparative HPLC (RP18, CH₃CN—H₂O).

Yield: 2.5 mg (26% of the theoretical value), M/e=633.0

Example 10

The following compounds can be prepared analogously to the examples above:

Example 10 Name R2 R3 A (8S)-5-(3-pyridyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

B (8S)-5-(4-pyridyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

C (8S)-5-(5-indolyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

D (8S)-5-(4-dimethylaminophenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3,5′,8′(2H)-pentone

E (8S)-5-[4-(3,4-dimethylisoxazolyl)]-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3 -dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

F (8S)-5-(3-furyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

G (8S)-5-(4-benzyloxyphenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

H (8S)-5-(4-methoxyphenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

I (8S)-5-(2-thiophenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

J (8S)-5-(3-thiophenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

K (8S)-5-(4-carboxamidophenyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

L (8S)-5-(1-dibenzofuranoyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

M (8S)-5-(2-N-methylpyrrolyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

N (8S)-5-(2-pyridazinyl)-4′,9,9′-trihydroxy-6′-methoxy-3-[(1E,3E)-penta-1,3-dienyl]-6,7-dihydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-1,1′-3′,5′,8′(2H)-pentone

O (8S)-5-(phenyl)-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-carbaldehyde O-methyloxime

P (8S)-5-(2-thiophenyl)-4′,9,9′-trihydroxy-6′-methoxy-1,1′,3′,5′,8′-pentaoxo-1,1′,2,3′,5′,6,7,8′-octahydrospiro[cyclopenta[g]isoquinoline-8,2′-cyclopenta[b]-naphthalene]-3-carbaldehyde O-methyloxime

Example 11 Water Solubility of the Fredericamycin Derivatives

The water solubility of the various fredericamycin derivatives can be determined in 0.9% NaCl solution with a pH of 7. 

1. A compound according to the general formula Ia or Ib:

wherein in each, R1 is H, C₁-C₆ alkyl, cycloalkyl, or C₁-C₄ alkylcycloalkyl; R2 is C₁-C₁₄ alkyl, C₂-C₁₄ alkenyl, 1,3-butadienyl, 1-butane, C₁-C₄ alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, cycloalkyl, C₁-C₄ alkyl-cycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, C_(m)H_(2m+o−p) Y″_(p), CH₂NHCOR21, CH₂NHCSR21, CH₂S(O)nR21, with n=0, 1, 2, CH₂SCOR21, CH₂OSO₂—R21, CHO, CH═NOH, CH(OH)R21, —CH═NOR21, —CH═NOCOR21, —CH═NOCH₂CONR21R22, —CH═NOCH(CH₃)CONR21R22, —CH═NOC(CH₃)₂CONR21R22, —CH═N—NHCO—R23, —CH═N—NHCO—CH₂NHCOR21, —CH═N—O—CH₂NHCOR21, —CH═N—NHCS—R23, —CH═CR24R25 (trans or cis), COOH, COOR21, CONR21R22, —CH═NR21, —CH═N—NR21R22,

—CH═N—NHSO₂ aryl, or —CH═N—NHSO₂ heteroaryl, wherein m is 1 to 6, o is 1, p is 1 to 2m+o; m is 2 to 6, o is −1, p is 1 to 2m+o; or m is 4 to 6, o is −2, p is 1 to 2m+o; Y″ is independently from each other selected from the group consisting of halogen, OH, OR21, NH₂, NHR21, NR21R22, SH and SR21; and wherein X′ is NR215, O, or S; and R211, R212, R213, R214, R215 are independently from each other H or C₁-C₆ alkyl; R21, R22 are independently from each other C₁-C₁₄ alkyl, C₁-C₁₄ alkanoyl, C₁-C₆ alkylhydroxy, C₁-C₆ alkylamino, C₁-C₆alkylamino-C₁-C₆ alkyl, C₁-C₆ alkylamino-di-C₁-C₆ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, aryl, aryloyl, C₁-C₄ alkylaryl, heteroaryl, heteroaryloyl, C₁-C₄ alkylheteroaryl, cycloalkanoyl, C₁-C₄ alkanoylcycloalkyl, heterocycloalkanoyl, C₁-C₄ alkanoylheterocycloalkyl, C₁-C₄ alkanoylaryl, C₁-C₄ alkanoylheteroaryl, or mono- and di-sugars linked through a C atom which would carry an OH group in the sugar, wherein the sugars are independently from each other selected from the group consisting of glucuronic acid and its stereo isomers at all optical atoms, aldopentoses, and aldohexoses, including their desoxy compounds; R23 independently of R21, is R21, a CH₂-pyridinium salt, or a CH₂-tri-C₁-C₆ alkylammonium salt; R24 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; R25 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; or R24, R25 together are C₄-C₈ cycloalkyl; R3 is C₂-C₁₄ alkyl, C₂-C₁₄ alkenyl, C₂-C₁₄ alkinyl, aryl, C₁-C₄ alkylaryl, heteroaryl, C₁-C₄ alkylheteroaryl, wherein the aryls or heteroaryls may be substituted with another aryl, C₁-C₄ alkylaryl, O-aryl, C₁-C₄ alkyl-O-aryl, heteroaryl, C₁-C₄ alkylheteroaryl, O-heteroaryl or C₁-C₄ alkyl-O-heteroaryl, cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, C_(m)H_(2m+o−p)Y′_(p), CH₂NHCOR31, CH₂NHCSR31, CH₂S(O)nR31, CH₂SCOR31, CH₂OSO₂—R31, CHO, CH═NOH, CH(OH)R31, —CH═NOR31, —CH═NOCOR31, —CH═NOCH₂CONR31R32, —CH═NOCH(CH₃)CONR31R32, —CH═NOC(CH₃)₂CONR31R32, —CH═N—NHCO—R33, —CH═N—NHCO—CH₂NHCOR31, —CH═N—O—CH₂NHCOR31, —CH═N—NHCS—R33, —CH═CR34R35 (trans or cis), COOH, COOR31, CONR31R32, —CH═NR31, —CH═N—NR31R32,

(with X′ ═NR315, O, S, and R311, R312, R313, R314, R315 being independently from each other H or C₁-C₆ alkyl), —CH═N—NHSO₂ aryl, or —CH═N—NHSO₂-heteroaryl, wherein m is 2-6, o is 1 or −1, and p is 1 to 2m+o; or m is 4-6, o is −3 and p is 1 to 2m+o; and Y′ is independently from each other selected from the group consisting of halogen, OH, OR31, NH2, NHR31, NR31R32, SH, and SR31; and wherein n is 0, 1 or 2; R31, R32 mean independently from each other C₁-C₁₄ alkyl, C₁-C₁₄ alkanoyl, C₁-C₆ alkylhydroxy, C₁-C₆ alkylamino, C₁-C₆ alkylamino-C₁-C₆ alkyl, C₁-C₆ alkylamino-di-C₁-C₆ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, aryl, aryloyl, C₁-C₄ alkylaryl, heteroaryl, heteroaryloyl, C₁-C₄ alkylheteroaryl, cycloalkanoyl, C₁-C₄ alkanoylcycloalkyl, heterocycloalkanoyl, C₁-C₄ alkanoylheterocycloalkyl, C₁-C₄ alkanoylaryl, C₁-C₄ alkanoylheteroaryl, alkanoylaryl, C₁-C₄ alkanoylheteroaryl, or mono- and di-sugars linked through a C atom which would carry an OH group in the sugar, wherein the sugars are independently from each other selected from the group consisting of glucuronic acid and its stereo isomers at all optical atoms, aldopentoses, and aldohexoses, including their desoxy compounds; R33 independently of R31, is R31, a CH₂-pyridinium salt, or a CH₂-tri-C₁-C₆ alkylammonium salt; R34 independently of R21, is R31, H, CN, COCH₃, COOH, COOR21, CONR31R32, NH₂, or NHCOR31; or R35 independently of R31, is R31, H, CN, COCH₃, COOH, COOR31, CONR31R32, NH₂, or NHCOR31; R34, R35 together are C₄-C₈ cycloalkyl; R5 is H, C₁-C₆ alkyl, cycloalkyl, C₁-C₄ alkylcycloalkyl, heterocycloalkyl, C₁-C₄ alkylheterocycloalkyl, aryl, C₁-C₄ alkylaryl, heteroaryl, or C₁-C₄ alkylheteroaryl; R4, R6, R7 independently from each other are H, C₁-C₆ alkyl, or CO—R41; R41 independently of R21, is R21; X is O, S, NH, or N—R8, wherein R8 independently from R5 is R5, or R5 and R8, together with the N, form a ring with 4, 5, 6, 7, or 8 members, which may optionally contain still another heteroatom selected from the group consisting of N, O, and S; or X—R5 may together be H; Y is O, S, or NR9, wherein R9 is H or C₁-C₆ alkyl; or a stereoisomer, tautomer or physically tolerable salt thereof.
 2. The compounds according to claim 1, wherein Formula Ia or Ib adopt the stereochemistry of Formula IIa or IIb


3. The compound according to claim 1, wherein R1 is H, C₁-C₅ alkyl, or cycloalkyl; R2 is C₁-C₅ alkyl, C₁-C₄ alkylaryl, C₂-C₅ alkenyl, heteroaryl, C₁-C₄ alkylheteroaryl, CHF₂, CF₃, polyol side chain, CHOH—CHOH—CHOH—CHOH—CH₃, CHOH—CHOH—CH═CH—CH₃, CH═CH—CHOH—CHOH—CH₃, CH₂Y′″(Y′″═F, Cl, Br, I), CH₂NH₂, CH₂NR21R22, CH₂NHCOR23, CH₂NHCSR23, CH₂SH, CH₂S(O)nR21, with n=0, 1, 2, CH₂SCOR21, CH₂OH, CH₂OR21, CH₂OSO₂—R21, CHO, CH(OR21)₂, CH(SR21)₂, CN, CH═NOH, CH═NOR21, CH═NOCOR21, CH═N—NHCO—R23, CH═CR24, R25 (trans or cis), COOH, COOR21, CONR21R22, —CH═NR21, —CH═N—NR21R22,

—CH═N—NHSO₂-aryl, —CH═N—NHSO₂-heteroaryl, or wherein X′ is NR215, O, or S; and R211, R212, R213, R214, and R215 are independently from each other are H or C₁-C₆ alkyl; R21, R22 independently from each other are C₁-C₆ alkyl, cycloalkyl, aryl, C₁-C₄ alkylaryl, heteroaryl, or C₁-C₄ alkylheteroaryl; R23, independently of R21, is R21, a CH₂-pyridinium salt, or a CH₂-tri-C₁-C₆ alkylammonium salt; R24 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; R25 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; or R24, R25 together are C₄-C₈ cycloalkyl; R3 is C₂-C₁₄ alkyl, C₂-C₁₄ alkenyl, C₂-C₁₄ alkinyl, aryl, C₁-C₄ alkylaryl, heteroaryl or C₁-C₄ alkylheteroaryl, wherein the aryls or heteroaryls may be substituted with another aryl, C₁-C₄ alkylaryl, O-aryl, C₁-C₄ alkyl-O-aryl, heteroaryl, C₁-C₄ alkylheteroaryl, O-heteroaryl or C₁-C₄ alkyl-O-heteroaryl; R5 is H, C₁-C₃ alkyl, or cycloalkyl; R4, R6, R7 independently from each other are H, C₁-C₅ alkyl, or CO—R41; R41 independently of R21, is R21; X is O, S, NH, or N—R8; Y is O, S, or NH.
 4. The compound according to claim 1 in the form of an inclusion compound with cyclodextrin.
 5. The compound according to claim 2 wherein R1 is H, C₁-C₅ alkyl, or cycloalkyl; R2 is C₁-C₅ alkyl, C₁-C₄ alkylaryl, C₂-C₅ alkenyl, heteroaryl, C₁-C₄ alkylheteroaryl, CHF₂, CF₃, polyol side chain, CHOH—CHOH—CHOH—CHOH—CH₃, CHOH—CHOH—CH═CH—CH₃, CH═CH—CHOH—CHOH—CH₃, CH₂Y (Y′″═F, Cl, Br, I), CH₂NH₂, CH₂NR21R22, CH₂NHCOR23, CH₂NHCSR23, CH₂SH, CH₂S(O)nR21, with n=0, 1, 2, CH₂SCOR21, CH₂OH, CH₂OR21, CH₂OSO₂-R21, CHO, CH(OR21)₂, CH(SR21)₂, CN, CH═NOH, CH═NOR21, CH═NOCOR21, CH═N—NHCO—R23, CH═CR24, R25 (trans or cis), COOH,

COOR21, CONR21R22, —CH═NR21, —CH═N—NR21R22, —CH═N—NHSO₂-aryl, —CH═N—NHSO₂-heteroaryl, or CH═N—NHC—R23, wherein X′ is NR215, O, or S; and R211, R212, R213, R214, and R215 are independently from each other are H or C₁-C₆ alkyl; R21, R22 independently from each other are C₁-C₆ alkyl, cycloalkyl, aryl, C₁-C₄ alkylaryl, heteroaryl, or C₁-C₄ alkylheteroaryl; R23 independently of R21, is R21, a CH₂-pyridinium salt, or a CH₂-tri-C₁-C₆ alkylammonium salt; R24 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; R25 independently of R21, is R21, H, CN, COCH₃, COOH, COOR21, CONR21R22, NH₂, or NHCOR21; or R24, R25 together are C₄-C₈ cycloalkyl; R3 is C₂-C₁₄ alkyl, C₂-C₁₄ alkenyl, C₂-C₁₄ alkinyl, aryl, C₁-C₄ alkylaryl, heteroaryl, or C₁-C₄ alkylheteroaryl, wherein the aryls or heteroaryls may be substituted with another aryl, C₁-C₄ alkylaryl, O-aryl, C₁-C₄ alkyl-O-aryl, heteroaryl, C₁-C₄ alkylheteroaryl, O-heteroaryl or C₁-C₄ alkyl-O-heteroaryl; R5 is H, C₁-C₃ alkyl, or cycloalkyl; R4, R6, R7 independently from each other are H, C₁-C₅ alkyl, or CO—R41; R41 independently of R21, is R21; X is O, S, NH, or N—R8; Y is O, S, or NH.
 6. A pharmaceutical composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier or adjuvant.
 7. A pharmaceutical composition comprising a compound of claim 2 and a pharmaceutically acceptable carrier or adjuvant. 